Reporter

Part:BBa_K1676051:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-16)


GFP Reporter with Mutant RBS 5AT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


Design Notes

pLac was used as it is a constitutive promoter in S. Oneidensis MR1.


Source

The basic parts are from the iGEM registry. Consisting of the pLac promoter BBa_R0011, eGFP BBa_E0040, double terminator BBa_B0015, we ligated the parts( including original wild type RBS) together and perform site targeted mutagenesis on the construct to mutate the WT RBS.

References